论文标题：Constructing vesicle-based artificial cells with embedded living cells as organelle-like modules

期刊：Scientific Reports

作者：Yuval Elani, Tatiana Trantidou, Douglas Wylie, Linda Dekker, Karen Polizzi, Robert V. Law, Oscar Ces

发表时间：2018/03/14

数字识别码：10.1038/s41598-018-22263-3

原文链接：

https://www.nature.com/articles/s41598-018-22263-3?utm_source=Other_website&utm_medium=Website_links&utm_content=RenLi-MixedBrand-multijournal-Multidisciplinary-China&utm_campaign=ORG_USG_JRCN_RL_article_promotion_sciencenet_Oct_3rd

将生物和合成功能赋予脂质囊泡，使其成为人工细胞是一个日渐热门的研究领域。但是与生物细胞相比，人工细胞的能力有限，因为它们缺乏活细胞具有的复杂性和在进化中形成的生化通道。

在《科学报告》发表的一篇名为Constructing vesicle-based artificial cells with embedded living cells as organelle-like modules的文章里，来自英国伦敦帝国学院化学系的Oscar Ces的团队提出了制造人工细胞的新策略。他们将活细胞封装在囊泡中，令人工细胞能利用活细胞的生物化学特征，而活细胞在整个体系中充当了细胞器的作用。作者使用微流体技术构建了这种混合细胞仿生系统，并证明了囊泡宿主和包封的活细胞能协同工作。囊泡的外部结构保护活细胞免受有毒环境的影响，而细胞充当生物反应器模块，处理包封的原料，并通过共同包封在囊泡中的合成酶代谢系统进行进一步加工。

图1：把活细胞封装在囊泡内

摘要：There is increasing interest in constructing artificial cells by functionalising lipid vesicles with biological and synthetic machinery. Due to their reduced complexity and lack of evolved biochemical pathways, the capabilities of artificial cells are limited in comparison to their biological counterparts. We show that encapsulating living cells in vesicles provides a means for artificial cells to leverage cellular biochemistry, with the encapsulated cells serving organelle-like functions as living modules inside a larger synthetic cell assembly. Using microfluidic technologies to construct such hybrid cellular bionicsystems, we demonstrate that the vesicle host and the encapsulated cell operate in concert. The external architecture of the vesicle shields the cell from toxic surroundings, while the cell acts as a bioreactor module that processes encapsulated feedstock which is further processed by a synthetic enzymatic metabolism co-encapsulated in the vesicle.

阅读论文全文请访问：

https://www.nature.com/articles/s41598-018-22263-3?utm_source=Other_website&utm_medium=Website_links&utm_content=RenLi-MixedBrand-multijournal-Multidisciplinary-China&utm_campaign=ORG_USG_JRCN_RL_article_promotion_sciencenet_Oct_3rd

期刊介绍：Scientific Reports (https://www.nature.com/srep/) is an online, open access journal from the publishers of Nature. We publish scientifically valid primary research from all areas of the natural and clinical sciences.

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（来源：科学网）