论文标题：Demonstration of End-to-End Automation of DNA Data Storage

期刊：Scientific Reports

作者：Christopher N. Takahashi, Bichlien H. Nguyen, Karin Strauss, Luis Ceze

发表时间：2019/03/21

数字识别码：10.1038/s41598-019-41228-8

原文链接：http://t.cn/EScQ2XI

合成DNA已经成为一种用于计算机数据编码的新型载体，其储存密度要比当代尖端技术高出几个数量级。然而，即使借助自动合成和测序设备，很多中间步骤仍然需要专业的实验室技术人员来执行。近期发表在Scientific Reports的研究Demonstration of End-to-End Automation of DNA Data Storage展示了一种新开发的自动化端到端DNA存储设备，研究人员希望由此探索在这种独特应用程序的约束下要想实现自动化会面临哪些挑战。

使用这个新设备可将数据编码为DNA序列，然后使用定制的DNA合成仪将其写入DNA寡核苷酸，之后将这些寡核苷酸集中起来以液体形式储存，并使用纳米孔测序仪和一种新颖的、准备工作最少的方案进行读取。研究人员在文章中展示了自动化的5字节写入、储存和读取周期，该周期采用了模块化设计，随着新技术的不断推出可实现进一步的扩展。

图1：写入、存储和读取流程示意图

摘要：However, even with the help of automated synthesis and sequencing devices, many intermediate steps still require expert laboratory technicians to execute.

We have developed an automated end-to-end DNA data storage device to explore the challenges of automation within the constraints of this unique application. Our device encodes data into a DNA sequence, which is then written to a DNA oligonucleotide using a custom DNA synthesizer, pooled for liquid storage, and read using a nanopore sequencer and a novel, minimal preparation protocol. We demonstrate an automated 5-byte write, store, and read cycle with a modular design enabling expansion as new technology becomes available.

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期刊介绍：Scientific Reports (https://www.nature.com/srep/) is an online, open access journal from the publishers of Nature. We publish scientifically valid primary research from all areas of the natural and clinical sciences.

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（来源：科学网）