2026年10月29日，美国怀特海生物医学研究所Kutay Deniz Atabay等科学家在《科学》（Science）发表研究，构建了成年脊椎动物Danionella cerebrum的全身单细胞图谱。

一个完整的脊椎动物细胞类型转录组图谱可促进对动物细胞类型组成、组织和演化的理解。小型化、透明且具有再生能力的真骨鱼Danionella cerebrum使全脊椎动物单细胞分析实验成为可能。研究人员对成年Danionella全身进行了区域分层单细胞RNA测序，并利用空间转录组学以单细胞分辨率绘制了细胞类型和基因表达图谱。他们根据区域基因表达特征描绘了空间上不同的神经细胞类型。该全身图谱阐明了携带成年分子位置记忆的细胞类型，揭示了幼态延续特征，并揭示了成年结缔组织中保守的身体区域和附肢特化程序。比较分析揭示了保守的神经细胞类型，再生数据集揭示了端脑再生过程中的表达动态。这个全脊椎动物转录组图谱为生物学和神经科学中的众多问题提供了全面资源。

附：英文原文

Title: Whole-body single-cell atlas of an adult vertebrate, Danionella cerebrum

Author: Kutay Deniz Atabay, Patrick Aoude, Chanyoung Park, Mykola Kadobianskyi, Olivier H. Paugois, Benjamin Judkewitz, Peter W. Reddien

Issue&Volume: 2026-10-29

Abstract: A complete cell type transcriptome atlas of a vertebrate could promote understanding of animal cell type composition, organization, and evolution. The miniaturized, transparent, and regenerative teleost Danionella cerebrum brings whole-vertebrate single-cell profiling experiments within reach. We performed region-stratified single-cell RNA sequencing across the adult Danionella body and mapped cell types and gene expression at single-cell resolution using spatial transcriptomics. We delineated spatially distinct neural cell types on the basis of their regional gene expression signatures. The body-wide atlas elucidated cell types harboring adult molecular positional memory, uncovered paedomorphic features, and revealed conserved body-region and appendage-specification programs in adult connective tissue. Comparative analyses revealed conserved neural cell types, and regeneration datasets uncovered expression dynamics during telencephalon regeneration. This whole-vertebrate transcriptome atlas yields a comprehensive resource for myriad questions in biology and neuroscience.

DOI: 10.1126/science.aeg1125

Source:https://www.science.org/doi/10.1126/science.aeg1125