韩国浦项科技大学Sung Ho Ryu等合作开发出用于长期活细胞单个蛋白成像的超高光稳定有机染料。相关论文于2025年1月15日发表于国际顶尖学术期刊《自然—方法学》杂志上。

该团队介绍了一种超高光稳定性和高亮度的有机染料——Phoenix Fluor 555 (PF555)，其光漂白寿命比传统有机染料长一个数量级，并且不需要任何抗光漂白添加剂。PF555 是一种非对称的氰染料，其结构中将传统氰染料Cyanine-3中的吲哚基团的一侧替换为3-氧-喹啉。

PF555为长期活细胞单分子成像提供了强大的工具，例如在生理条件下成像表皮生长因子受体（EGFR）与细胞膜上网格蛋白涂层结构的动态单分子相互作用。这展示了 PF555 在实时活细胞成像中的巨大潜力。

研究人员表示，有机染料具有光稳定性和高亮度等优点，在活细胞成像中起着至关重要的作用。

附：英文原文

Title: Super-photostable organic dye for long-term live-cell single-protein imaging

Author: Kim, Do-Hyeon, Triet, Hong Minh, Lee, Sun Hyeok, Jazani, Sina, Jang, Seongjae, Abedi, Syed Ali Abbas, Liu, Xiaogang, Seo, Jongcheol, Ha, Taekjip, Chang, Young-Tae, Ryu, Sung Ho

Issue&Volume: 2025-01-15

Abstract: Organic dyes play a crucial role in live-cell imaging because of their advantageous properties, such as photostability and high brightness. Here we introduce a super-photostable and bright organic dye, Phoenix Fluor 555 (PF555), which exhibits an order-of-magnitude longer photobleaching lifetime than conventional organic dyes without the requirement of any anti-photobleaching additives. PF555 is an asymmetric cyanine structure in which, on one side, the indole in the conventional Cyanine-3 is substituted with 3-oxo-quinoline. PF555 provides a powerful tool for long-term live-cell single-molecule imaging, as demonstrated by the imaging of the dynamic single-molecule interactions of the epidermal growth factor receptor with clathrin-coated structures on the plasma membrane of a live cell under physiological conditions.

DOI: 10.1038/s41592-024-02584-0

Source:https://www.nature.com/articles/s41592-024-02584-0