北京大学徐成冉课题组实现单细胞分辨率下的内胚层器官发生的时空和遗传细胞谱系追踪。2025年1月16日，《细胞》杂志在线发表了这项成果。

研究人员介绍了使用新型小鼠品系为14个不同内胚层区域设计的创新遗传追踪代码。通过将高通量和高精度的单细胞RNA测序与复杂的成像技术相结合，研究人员详细描述了内胚层在单细胞分辨率下的时空和遗传谱系分化。研究人员发现了早期内胚层区域内意外的多潜能性，能够分化成各种器官原基。

这项研究揭示了一个复杂且被低估的现象，即内胚层器官来自多个来源的发育，促使人们重新评估传统的分化模型。我们的发现推动了发育生物学的理解，并对再生医学和先进类器官模型的开发具有重要意义，为指导器官发生的复杂机制提供了深刻的见解。

研究人员表示，在早期哺乳动物发育过程中，内胚层胚层通过复杂的模式化形成呼吸系统和消化系统的基础。这个由一系列细胞命运决定引导的复杂过程仍然只有部分被理解。

附：英文原文

Title: Spatiotemporal and genetic cell lineage tracing of endodermal organogenesis at single-cell resolution

Author: Ke-Ran Li, Pei-Long Yu, Qi-Qi Zheng, Xin Wang, Xuan Fang, Lin-Chen Li, Cheng-Ran Xu

Issue&Volume: 2025-01-16

Abstract: During early mammalian development, the endoderm germ layer forms the foundation of the respiratory and digestive systems through complex patterning. This intricate process, guided by a series of cell fate decisions, remains only partially understood. Our study introduces innovative genetic tracing codes for 14 distinct endodermal regions using novel mouse strains. By integrating high-throughput and high-precision single-cell RNA sequencing with sophisticated imaging, we detailed the spatiotemporal and genetic lineage differentiation of the endoderm at single-cell resolution. We discovered an unexpected multipotentiality within early endodermal regions, allowing differentiation into various organ primordia. This research illuminates the complex and underestimated phenomenon where endodermal organs develop from multiple origins, prompting a reevaluation of traditional differentiation models. Our findings advance understanding in developmental biology and have significant implications for regenerative medicine and the development of advanced organoid models, providing insights into the intricate mechanisms that guide organogenesis.

DOI: 10.1016/j.cell.2024.12.012

Source: https://www.cell.com/cell/abstract/S0092-8674(24)01425-9